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HI-83303K Aquaculture Photometer kit

HI-83303K Aquaculture Photometer Kit

Kit contains:

  • HI 83303-02 - meter
  • HI 93700-01 - ammonia tests x 100
  • HI 93732-01 - dissolved oxygen tests x 100
  • HI 93710-01 - pH tests x 100
  • HI 93728-01 - nitrate tests x 50
  • HI 93707-01 - nitrite tests x 50
  • HI 740222N - blue carrying case

Advanced optical system - Unparalleled performance from a benchtop photometer

One meter can be used for both photometric and pH measurements. The meter has 20 different programmed methods measuring 12 key water quality parameters and also offers an absorbance measurement mode for performance verification and for users who would like to develop their own concentration versus absorbance curves. To save valuable benchtop space, the HI-83303 doubles as a professional pH meter with its digital pH/temperature electrode input. Aquaculture parameters include Alkalinity, Calcium, Nitrite, and Phosphate which are critical to maintaining a healthy system. It also contains parameters specific to either a marine or freshwater environment.

The photometer features an innovative optical system that uses LEDs, narrow band interference filters, focusing lens and both a silicon photodetector for absorbance measurement and a reference detector to maintain a consistent light source. It ensures accurate and repeatable photometric readings every time.

Made with the aquaculture industry in mind, the HI-83303 is a comprehensive solution to maintaining optimal chemical and environmental conditions, preventing disease and increasing production.

The HI-83303 measures vital parameters such as Alkalinity, Calcium, Nitrite, and Phosphate. Alkalinity plays a part in a dynamic relationship with pH and CO2concentrations, high alkalinity water lowers fluctuations in pH. The buffering capacity acts to store extra CO2 essential for photosynthesis in the ponds to produce oxygen. Maintaining calcium at certain levels is vital to proper fish growth and development. Excessive nitrite can be toxic to fish. When nitrite interacts with Haemoglobin, the iron becomes oxidised and the blood cell can no longer carry oxygen. Phosphate is essential to plant growth; too much phosphate in an aquaculture system can contribute to algal blooms decreasing dissolved oxygen vital for a successful ecosystem.

A digital pH electrode input allows the user to measure pH by a traditional glass electrode.

The HI-83303 offers an absorbance measuring mode that allows for CAL Check standards to be used to validate the performance of the system. The absorbance mode allows the user to select one of the five wavelengths of light (420 nm, 466 nm, 525 nm, 575 nm, and 610 nm) to measure and plot their own concentration versus absorbance mode. This is useful for users with their own chemical method and for educators to teach the concept of absorbance by using the Beer-Lambert Law.

Two USB ports are provided for transferring data to a flash drive or computer and to use as a power source for the meter. For added convenience and portability the meter can also operate on an internal 3.7 VDC Lithium-polymer rechargeable battery.

High Efficiency LED Light Source

An LED light source offers superior performance as compared to a tungsten lamp. LEDs have a much higher luminous efficiency, providing more light while using less power. They also produce very little heat, which could otherwise affect the optical components and electronic stability. LEDs are available in a wide array of wavelengths, whereas tungsten lamps are supposed to be white light (all wavelengths of visible light) but actually have a poor blue/violet light output.

High Quality Narrow Band Interference Filters

The narrow band interference filter not only ensure greater wavelength accuracy (+/- 1 nm) but are extremely efficient. The filters used allow up to 95% of the light from the LED to be transmitted as compared to other filters that are only 75% efficient. The higher efficiency allows for a brighter, stronger light source. The end result is higher measurement stability and less wavelength error.

Reference Detector for a Stable Light Source

A beam splitter is used as part of the internal reference system of the HI-83300 photometer. The reference detector compensates for any drift due to power fluctuations or ambient temperature changes. Now you can rely on a stable source of light between your blank (zero) measurement and sample measurement.

Large Cuvette Size

The sample cell of the HI-83303 fits a round, glass cuvette with a 25 mm path length. Along with the advanced optical components, the larger size of the cuvette greatly reduces errors in rotation from the indexing mark of the cuvettes. The relatively long path length of the sample cuvette allows the light to pass through more of the sample solution, ensuring accurate measurements even in low absorbance samples.

Focusing Lens for Greater Light Yield

Adding a focusing lens to the optical path allows for the collection of all of the light that exits the cuvette and focusing the light on the silicon photo detector. This novel approach to photometric measurements cancels the errors from imperfections and scratches present in the glass cuvette eliminating the need to index the cuvette.

Key features:

  • Advanced optical system with brighter, long-lasting LED light source
  • Backlit 128 x 64 Pixel Graphic Liquid Crystal Display
  • Built-in Reaction Timer for Photometric Measurements
  • Absorbance mode - concentration versus absorbance
  • Units of Measurement plus chemical form displayed
  • Result conversion at the touch of a button
  • Cuvette cover
  • pH and temperature measurement with a single probe
  • Good Laboratory Practice (GLP) - track calibration information including date, time, buffers used, offset and slope for traceability
  • CAL Check alerts user to potential problems during the calibration process
  • Data Logging - Up to 1000 photometric and pH readings can be stored
  • Logged readings can be quickly and easily transferred to a flash drive or to a PC. Data is exported as a .CSV file for use with spreadsheet programs
  • Battery Status Indicator
  • Error Messages


Specifications list


Input Channels 1 pH electrode input and 5 photometer wavelengths
pH Electrode Digital pH electrode (not included)
Logging type Log on demand with user name and sample ID optional input
Logging Memory 1000 readings
Connectivity USB-A host for flash drive; micro-USB-B for power and computer connectivity
GLP Calibration data for connected pH electrode
Display 128 x 64 pixel LCD with backlight
Battery Type/Life 3.7 VDC Li-polymer rechargeable battery/ >500 photometric measurements or 50 hours of continuous pH measurement
Power supply 5 VDC USB 2.0 power adapter with USB-A to micro-USB-B cable (included)
Environment 0 to 50.0°C (32 to 122.0°F; 0 to 95% RH, non-condensing
Dimensions 206 x 177 x 97mm (8.1 x 7.0 x 3.8")
Weight 1.0 kg (2.2lbs)
Photometer/Colorimeter Light Source 5 LEDs with 420nm, 466nm, 525nm, 575nm, and 610nm narrow band interference filters
Photometre/Colorimeter Light Detector Silicon Photodetector
Bandpass filter Bandwidth 8nm
Bandpass filter wavelength accruacy +-1nm
Cuvette Type Round, 24.6mm
Number of Methods 128 Max
Ordering Information HI-83303 is supplied with sample cuvettes and caps (x4), cloth for wiping cuvettes, USB to micro USB cable connector, power adapter and instruction manual.
Alkalinity Range Freshwater: 0 to 500 mg/L (as CaCO33)
Alkalinity Resolution 1 mg/L
Alkalinity Accuracy ±5 mg/L ±5% of reading
Alkalinity Method Colorimetric method
Ammonia Range Low Range: 0.00 to 3.00 mg/L (as NH3-N) Medium Range: 0.00 to 10.00 mg/L (as NH3-N) High Range: 0.0 to 100.0 mg/L (as NH3-N)
Ammonia Resolution 0.01 mg/L; 0.1 mg/L
Ammonia Accuracy Low Range: ±0.04 mg/L ±4% of reading Medium Range: ±0.05 mg/L ±5% of reading High range: ±0.5 mg/L ±5% of reading at 25 °C
Ammonia Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method
Calcium Range Freshwater: 0 to 400 mg/L (as Ca2+) Marine: 200 to 600 mg/L (as Ca2+)
Calcium Resolution 1 mg/L
Calcium Accuracy Freshwater: ±10 mg/L ±5% of reading Marine: ±6% of reading
Calcium Method Freshwater: Adaptation of the Oxalate method Seawater: Adaptation of the Zincon method
Free Chlorine Range 0.00 to 5.00 mg/L (as Cl2)
Free Chlorine Resolution 0.01 mg/L
Free Chlorine Accuracy ±0.03 mg/L ±3% of reading
Total Chlorine Range 0.00 to 5.00 mg/L (as Cl2)
Total Chlorine Resolution 0.01 mg/L
Total Chlorine Accuracy ±0.03 mg/L ±3% of reading
Chlorine Method Adaptation of the EPA 330.5 DPD method
Copper Range Low Range: 0.000 to 1.500 mg/L (as Cu2+) High range: 0.00 to 5.00 mg/L (as Cu2+)
Copper Resolution 0.001 mg/L; 0.01 mg/L
Copper Accuracy Low Range: ±0.01 mg/L ±5% of reading High Range ±0.02 mg/L ±4% of reading
Copper Method Adaptation of the EPA bicinchoninate method
Nitrate Range 0.0 to 30.0 mg/L (as NO3-- N)
Nitrate Resolution 0.1 mg/L
Nitrate Accuracy ±0.5 mg/L ±10% of reading
Nitrate Method Adaptation of the cadmium reduction method
Nitrite Range Freshwater Low Range: 0 to 600 μg/L (as NO2--N) High Range: 0 to 150 mg/L (as NO2-) Marine Ultra Low Range: 0 to 200 μg/L (as NO2--N)
Nitrite Resolution Freshwater: 1 μg/L; 1 mg/L Marine: 1 μg/L
Nitrite Accuracy Freshwater Low Range: ±20 μg/L ±4% of reading High Range: ±4 mg/L ±4% of reading Marine: ±10 μg/L ±4% of reading
Nitrite Method Adaptation of the EPA Diazotization method 354.1; HR Ferrous Sulphate
Oxygen, Dissolved Range 0.0 to 10.0 mg/L (as O2)
Oxygen, Dissolved Resolution 0.1 mg/L
Oxygen, Dissolved Accuracy ±0.4 mg/L ±3% of reading
Oxygen, Dissolved Method Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Azide modified Winkler method
pH Range Photometer: 6.5 to 8.5 pH pH electrode: -2.00 to 16.00 pH
pH Resolution Photometer: 0.1 pH pH electrode: 0.1 pH
pH Accuracy Photometer: ±0.1 pH pH electrode: ±0.01 pH
pH Calibration Automatic one or two point calibration with one set of standard buffers available (4.01, 6.86, 7.01, 9.18, 10.01)
pH Temperature Compensation Automatic (-5.0 to 100.0 oC; 23.0 to 212.0 oF); limits reduced based on the pH electrode used
pH CAL Check (electrode diagnostics) clean electrode and check buffer/check probe displayed during calibration
pH Method Photometer: phenol red
pH-mV Range ±1000 mV
pH-mV Resolution 0.1 mV
pH-mV Accuracy ±0.2 mV
Phosphate Range Freshwater Low Range: 0.00 to 2.50 mg/L (as PO43-) High range: 0.0 to 30.0 mg/L (as PO43-) Seawater Ultra Low Range: 0 to 200 μg/L (as P)
Phosphate Resolution Freshwater: 0.01 mg/L; 0.1 mg/L Seawater: 1 μg/L
Phosphate Accuracy Freshwater Low Range: ±0.04 mg/L ±4% of reading High Range: ±1 mg/L ±4% of reading Seawater Ultra Low Range: ±5 μg/L ±5% of reading
Phosphate Method Freshwater Low Range: Adaptation of the Ascorbic Acid method Freshwater High Range and Seawater Ultra Low Range: Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Amino Acid method
Alkalinity HI-775-26 for freshwater; HI-755-26 for saltwater
Ammonia HR & LR HR - HI-93733-01 (100 tests); HI-93733-03 (300 tests); MR - HI-93715-01 (100 tests); HI-93715-03 (300 tests); LR - HI-93700-01 (100 tests); HI-93700-03 (300 tests)
Calcium HI-937521-01 (50 tests); HI-937521-03 (150 tests)
Calcium - Marine HI-758-26
Chlorine - Free Free Chlorine Reagents: HI-93701-01 (100 tests); HI-93701-03 (300 tests); HI-93701-F (liquid) (300 tests)
Chlorine Total HI-93711-01 (100 tests); HI-93711-03 (300 tests); HI-93711-T (300 tests liquid)
Copper – HR, total & LR HR - HI-93702-01 (100 tests); HI-93702-03 (300 tests)
Total copper - HI-93702T - 01 (100 tests); HI-93702T-03 (300 tests)
LR - HI-95747-01 (100 tests); HI-95747-03 (300 tests)
Nitrate HI-93728-01 (100 tests); HI-93728-03 (300 tests)
Nitrite – HR & LR HR - HI-93708-01 (100 tests); HI-93708-03 (300 tests)
LR - HI-93707-01 (100 tests); HI-93707-03 (300 tests)
Nitrite - ULR Marine HI-764-25
Oxygen, Dissolved (DO) HI-93732-01 (100 tests); HI-93732-03 (300 tests)
pH HI-93710-01 (100 tests); HI-93710-03 (300 tests)
Phosphate - ULR Marine HI-736-25
Phosphate - HR & LR HR - HI-93717-01 (100 tests); HI-93717-03 (300 tests)
LR - HI-93713-01 (100 tests); HI-93713-03 (300 tests)